E.Coli

KHUSHBU SHAH
BIOTECHNOLOGY
Tuesday July 15, 2009

Trans take a hoping Escherichia cells with plasmids containing Genes for GFP
* (citations written on copy I printed out, will them in for final)
ABSTRACT:
The purpose of the experimentation was to p poleuce common land fluorescence by taking Escherichia (E.coli) bacteria and mixing it with a protein plasmid. If E.coli bacteria is change with green fluorescent protein plasmids then the cells will glow green under UV light because the Eschericia bacteria will look at in green fluorescent protein producing green glow. In conclusion, the experiment was successful because the ordered series that contained the green fluorescent protein and ampicilin contained bacterial colonies.
initiation:
Bacterium is a round spiral or rod chassisd single celled microorganism that may lack a cell wall. It comes in rod, spiral or curved shape and uses hair called flagella to move around. An E.coli is a bacterium that is commonly base in the intestines of warm blooded organisms and has a circular shape. E.coli is an organism that does not naturally enter a state of readiness (standardized environment so an individual mickle perform a specific job). The Escherichia coli (E.



coli) was ideal for this transformation study because it can be easily grown in Luria broth (used for growth in bacteria)
or on an agar. Agar or agar plate is a solid environment where bacteria can grow. By allowing certain bacteria to grow is called selective medium. Bacteria can be grown in both liquid or a solid environment. To produce bacterial growth on solid environment the cell will first form a colony which is a combination of cells growing in the outward direction (1). The bacterial growth inside a solid environment is different from those in liquid because in solid environment one bacterium does not modulate the other bacterium therefore the cells that grow are neer in synch. In the laboratory, when mixing Calcium Chloride and Plasmid deoxyribonucleic acid and by placing this mixture on...If you want to get a profuse essay, order it on our website: Orderessay



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